All posts by robert01pd2014

Brotein

Since last year I have been supplementing my workouts with protein powder. I believe that the gains I have obtained while supplementing my workouts with protein powder were achieved at a faster rate than when I worked out and received all my nutrients from the food I ingested. Getting my protein powder at first was really difficult because my father was seriously against me putting “unnatural” supplements into my body. It took a little convincing but I manage to buy my first protein powder 1 year and a half ago. Without truly looking into the details of protein powders, I based my purchase off of the supplement store manager’s recommendation, and bought myself an “All Natural Whey Isolate” Protein Powder! This protein powder was more expensive than all of the other powders in the store (should have rang some bells), but the manager assured me that it’s because this protein powder is all “natural”. I know from a light research done in the past that whey protein powder is derived from milk, therefore I wonder if the production of protein powder involved anyother materials (other than milk) , and if so are they also naturally occurring or manufactured in some lab.

Whey is a bi-product of cheese and casein manufacture. When 100 L of milk is used to produce cheese and casein, generally 12 kg of cheese is made, along with 3kg of casein, and 87 L of whey. This bi product named whey is definitely not the same Whey protein powder, which I put in my protein shake. This whey is 6% solid, and is a greenish liquid, which looks and tastes unimaginably horrible. This is why in the past whey was used as pig food and fertilizer and sometimes the whey was simply discarded into the ocean.

Whey Protein Concentrate is produced using ultrafiltration. Ultrafiltration keeps in a liquid product retentate, which consists of any insoluble material or solutes greater than 20000 Da molecular weight. The rest of the whey (greenish liquid) passes through the membrane and is called the permeate. The permeate consists of most of the lactose and H2O originally found in the whey. The retentate, which consists of only 1-4% of the original whey inserted into the ultrafiltration, is then spray dried into a powder that consists of 35-85% protein depending on the intending customer.

Digestive enzymes in a controlled environment manufacture whey Protein Hydrolysates, where the temperature and pH levels are controlled. Raw materials from Whey Protein Concentrate are used. The WPC raw materials are filtered and spray dried in the same way regular WPC are made, after which they are subjected to the digestive enzymes. A regular protein is a chain of amino acids in which the amine group of one amino acid is bound to the carboxylic group of a neighboring amino acid via a amide bond. Proteolytic enzymes catalyst the hydrolysis of these bonds. Chains of 2 to 5 amino acids are called peptides. In a hydrolysate, all the proteins are broken up into peptides and free amino acids no greater than the peptides.

Peptide Hydrolysis

Figure 1 Protein Hydrolysis

The research I performed has shed a new light on the substance I drink almost three times a day. This research has not only proven to me that chemistry is truly everywhere but has also shed some light onto the economical side of protein powder production. In conclusion the production of whey protein concentrate is “all natural” by that I mean that it involves only milk, which has been ultrafiltrated. The production of protein powder hydrolysate in my opinion is also “natural” because large amino acid chains are broken up using digestive enzymes, which are naturally occurring. The economical perspective of protein production comes from the fact that from 87 liters 3.48 liters of protein powder.

Protein Powder

“The Chemistry of Whey Protein – www.ChemistryIsLife.com.”www.ChemistryIsLife.com. N.p., n.d. Web. 8 Oct. 2013. <http://www.chemistryislife.com/the-chemistry-of-whey-protein>.

“Minimal whey protein with carbohydra… [Appl Physiol Nutr Metab. 2007] – PubMed – NCBI.” National Center for Biotechnology Information. N.p., n.d. Web. 8 Oct. 2013. <http://www.ncbi.nlm.nih.gov/pubmed/18059587>.


Anesthesia, Safe or Not?

Throughout my life I’ve had a couple of surgeries. Every time I have received surgery, my mother always asked the doctor, “Do you have to use general anesthesia?”. In times like that I have always told myself, why does my mom even ask, of course the doctors are going to use general anesthesia, why not?  One day I asked my mother “Why do you ask the doctors the same question every time?”, and she told me that general anesthesia was very dangerous, and if it is unnecessary it would be better not to use it. This is why I chose to research general anesthesia. I would like to formulate my own conclusions about the dangers of general anesthesia. The way I will do this is through a careful analysis of the facts, side effects and the way general anesthesia works. I will also use my knowledge of chemistry to aid me in making my conclusion.

The functions of anesthesia include: analgesia (no pain), amnesia (loss of consciousness), impairment of skeletal muscle, and weakened autonomic responses. The most important part of anesthesia is that after the surgery all of these effects can be reversed.  Not all anesthetics will provide all of these effects. For example, barbiturates are not analgesics, but will bring loss of consciousness. This is often why most anesthesias are a combination of many anesthetics.

Anesthetics have many side effects. The first one is a decrease in respiration. Anesthesiologists deal with this by attaching all patients to ventilators during surgery. The second most common side effect is nausea, when a patient is under general anesthesia, their lower esophageal sphincter is relaxed. To avoid death by aspiration, doctors use endotracheal tubes to provide ventilation.

Endotracheal tube

Picture 1: Endotracheal Tube

The third side effect is hypothermia. To prevent this side effect is one of the major goals for anesthesiologists. Anesthesiologists prevent hypothermia by warming the fluids (anesthesia), which are injected into the human body.

How does anesthesia work? Anesthetics block certain protein receptors, inhibiting the protein from performing its task. For example, the NMDA (N-methyl-D-aspartate) receptor is one of the main mediators of excitatory neurotransmission. The receptor is an ion channel, which permits the movement of calcium, sodium and potassium across the post-synaptic membrane. Anesthesiologists inhibit this protein receptor by depolarizing the cell with the anesthesia, which then results in the protein receptor not completing its task. Anesthesia depolarizes the cell inducing the cell with a net positive charge.  (Gambulos)

NMDA receptor

Picture 2: NMDA Receptor

The Ca2+ and Na+ enter the cell and induce a net increase of 3+. The anesthesias, which are involved, are Xenon, Ketamine (C13H16ClNO), and nitrous oxide (N2O). (Gambulos)

Anesthesias are really useful in putting you to “sleep”, but once surgery is over and you wake up you don’t want these chemicals to linger around in your body.This diagram shows how propofol (C12H18O) interacts with your metabolism in order to exit the human body.

Propofol and your metabolism

Picture 3: Propofol exiting the body

The propofol interacts with the liver glucuronate and sulfate conjugation. Then is excreted into the urine to exit the body. Usually 70% of the propofol is gone in 24 hours, and about 90% is gone in 5 days.

Before I started this blog assignment I thought anesthesia was the “simple” part of surgery. After doing all this research I have realized that the anesthesiologists must consider many things before using an anesthesia.  For example, if the anesthesia will interact correctly with the patients protein receptors. The anesthesiologist’s job doesn’t stop there, while the patient is “under” they must ensure that they are ready to deal with any side affects that might occur from the anesthesia, and after surgery is complete the anesthesiologist must then ensure that the anesthesia administered must exit the human body safely.

In conclusion, general anesthesia is safe. The side effects are all dealt with appropriately. For example, the hypothermia is dealt with warm anesthesia pumped through your veins. After examining the way anesthesia works, obstructing the function of protein receptors, I have realized that drugs work the same way. The reason why drugs are so dangerous is because often it is difficult to stay within the therapeutic window. Anesthesiologist deals with this by constantly staying by your side, ensuring that the drugs in your system do not exceed the toxic level or go under the therapeutic level. Finally the last part of anesthesia is the way it exits your body. Your body does this through a reaction of the anesthetics with your liver glucuronate and sulfate conjugation. Then the anesthetic proceeds to your urine. At first I thought this was dangerous, but then I remembered that almost every adult drinks alcohol, and 90% of the ethanol, from alcohol, is broken down by your liver. Therefore I concluded that the way anesthesia leaves your body is not so dangerous after all.

References

Garcia, Paul, Scott Kolesky, and Andrew Jenkins. “General Anesthetic Actions on GABAA Receptors .” PMC. Bentham Science Publishers, n.d. Web. 25 Mar 2013. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2866459/>.

Gambulos, Rachel. N.p., 20 04 2008. Web. 25 Mar 2013.

Gordon, G. (2010, August 31). Propofol-3. Retrieved from http://www.anesthesiawiki.net/metrohealthanesthesia/MHAnes/edu/ivanes/propofol3.htm

Lundbeck Institute. (n.d.). Nmda receptor, showing different subtypes. Retrieved from http://www.cnsforum.com/imagebank/item/hrl_rcpt_sys_NMDA/default.aspx

Oda, Yutaka. Hamoka, N. Hiroi, T., Imaoka, S., Hase, I., Tanaka K., Funae Y., Involvement of Human Liver Cytochrome P4502B6 in the metabolism of Propofol. The British Journal of Pharmacology. 51. 281-285. 2001.

Thomas, Shawn. Drug Reference for FDA Approved General Anesthetics @ Neurotransmitter.net. 2007.